Primer for detecting potato ralstonia solancearum and PCR detection method adopted by primer

The invention provides a primer for detecting potato ralstonia solancearum and a PCR detection method adopted by the primer, relating to a primer and a PCR detection method adopted by the primer, and aiming at solving the problems that when the existing methods are adopted for detecting pathogenic b...

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Bibliographische Detailangaben
Hauptverfasser:	LYU DIANQIU, DONG XUEZHI, GAO YUNFEI, YANG SHUAI, BAI YANJU, ZHANG SHU, WANG WENCHONG, MIN FANXIANG, WEI QI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a primer for detecting potato ralstonia solancearum and a PCR detection method adopted by the primer, relating to a primer and a PCR detection method adopted by the primer, and aiming at solving the problems that when the existing methods are adopted for detecting pathogenic bacteria of potato ralstonia solancearum, the detection range is narrow, the specificity is poor, the sensitivity is poor, and the detection efficiency is low. The primer has the sequence as follows: QK5-1: 5'-GCTAATACCGCATACGAC-3'; QK3-1: 5'-GAGCGTCAGTGTTATCCC-3'. The PCR detection method comprises the following steps: extracting the total genomic DNA, carrying out PCR detection, verifying the detection primer, and if an amplification band appears at the 591bp position, indicating that the sample is positive. According to the primer and the PCR detection method, the detection sensitivity can achieve 1pg of template DNA, the detection efficiency is high, the detected sample can be potato plant DNA and tuber DNA, eve