PCR identification method for rice typical abortion type and stained abortion type cytoplasmic male sterile lines

The invention discloses a PCR identification method for rice typical abortion type and stained abortion type cytoplasmic male sterile lines. According to the method, the total DNA of a to-be-tested rice material is divided into 3 parts, the 3 parts are respectively subjected to PCR amplification with a primer atp6-orf79, a primer orWA352 and a primer LD29; the PCR amplified products of the primer orWA352 and the primer LD29 are respectively single bands with 1432 bp and 2555 bp, the primer atp6-orf79 has no amplified band, and thus the detected material is the typical abortion type male sterile line; if the PCR amplified products of the primer atp6-orf79 and the primer LD29 are respectively single bands with 1592 bp and 905 bp and the primer orWA352 has no amplified band, the material is the stained abortion type male sterile line. The detected material can be pre-expected to be the typical abortion type or the stained abortion type in rice seed and seedling stages, and the operation is simple, accurate and f