HCV NS3 monoclonal antibodies and hepatitis C detection kit

The invention discloses a hybridoma cell strain HCV4, wherein the preservation number of the cell strain is CGMCC No.10120. The invention discloses a hybridoma cell strain HCV5, wherein the preservation number of the cell strain is CGMCC No.12282. Two monoclonal antibody hybridoma cell strains HCV4 and HCV5, for epitope 'MWTVYHGAG' and epitope 'TRKGSSGGP' of HCV NS3 N terminal, are prepared by the invention, and the two monoclonal antibodies, in the matched detection of an HCV NS3 antigen, are quite high in sensitivity and specificity. The monoclonal antibodies have an early detection advantage, namely infection in a patient can be detected within about one week. The monoclonal antibodies are high in sensitivity, and average detection sensitivity is 5.2pg/ml, which is much lower than ELISA detection method in the prior art which is 1-2ng/ml. 本发明公开了种杂交瘤细胞株HCV4，其中，所述细胞株的保藏编号为：CGMCC No.10120。杂交瘤细胞株HCV5，所述细胞株的保藏编号为：CGMCC No.12282。本发明制备得到的针对HCV NS3 N末端表位"MWTVYHGAG"和表位"TRKGSSGGP"两株单克隆抗体杂交瘤细胞株HCV4和HCV5，两单克隆抗体配对检测HCV