Method for changing roughness of crystalline basement by using soft lithography chip for protein crystallization

The invention provides a method for changing the roughness of a crystalline basement by using a soft lithography chip for protein crystallization. The method comprises the following steps: firstly, designing and producing a PDMS (Polydimethylsiloxane) chip meeting the crystallization requirements of protein; secondly, dropwise adding a protein crystallization solution on the surface of the PDMS chip, standing and crystallizing. According to the method provided by the invention, by designing the PDMS chip, a controllable crystallization interface with uniform roughness is realized, so that the nucleation probability of the protein on the surface of the basement is improved, and further the aim of improving the success rate of protein crystallization is achieved. According to the method, the screening success rate of protein crystallization conditions can be improved by 1 to 5 times. 本发明提供了种使用软刻芯片改变结晶基底粗糙度用于蛋白质结晶的方法，首先设计和制作满足蛋白质结晶要求的PDMS芯片，然后将蛋白质结晶溶液滴加在PDMS表面，静置结晶。本发明通过设计PDMS芯片实现可控和均粗糙度的结晶界面，来提高基底表面蛋白质形核几率，从而达到