Preparation method of animal testicle extract

The invention relates to the technical field of polypeptide extraction, in particular to a preparation method of an animal testicle extract. The preparation method of the animal testicle extract comprises the following steps: homogenizing an animal testicle, and conducting enzymatic hydrolysis and extraction, so that an enzymatically hydrolyzed crude extracting solution and filter residue are obtained; conducting ultra-filtration on the enzymatically hydrolyzed crude extracting solution, so that an ultrafiltrate is obtained, and removing high-molecular impurities of the ultrafiltrate by virtue of agarose gel hydrogen bond adsorption chromatography, so that an enzymatically hydrolyzed and extracted refined solution is obtained; extracting and concentrating the filter residues by virtue of ethanol, so that a concentrated alcohol extracting solution is obtained; and mixing the enzymatically hydrolyzed and extracted refined solution and the concentrated alcohol extracting solution, and conducting freeze-drying, s