A process for production of soluble sugars from biomass

The present invention provides a process for enzyme mediated hydrolysis of biomass for production of soluble sugars, wherein the said process comprises of steady addition of small portions of biomass to enzyme solution, enabling rapid solubilization of biomass. The process used for enzymatic saccharification allows for increased biomass loading, enzyme recycle and mitigation of substrate and product inhibitory effect. The recycling of unhydrolyzed biomass along with soluble enzyme ensures complete reuse of the said enzyme for effective repeated hydrolysis thereby increasing the overall productivity of enzyme used. 本发明提供用于生产可溶性糖的酶介导的生物质水解的方法，其中所述方法包括向酶溶液稳定添加小份的生物质，实现生物质的快速溶解。用于酶糖化的方法实现生物质载量增加、酶再循环以及底物和产物抑制效应的减轻。未水解的生物质与可溶性酶起再循环确保所述酶的完全重复用于有效的重复水解，由此增加所使用的酶的总体生产力。