Method for extracting high-purity neohesperidin from immature bitter orange

The invention discloses a method for extracting high-purity neohesperidin from an immature bitter orange. The method comprises the following steps: (1) crushing and impregnating an immature bitter orange raw material in water, adding a compound plant-hydrolase preparation, and performing enzymatic d...

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Bibliographische Detailangaben
Hauptverfasser: LONG WEIAN, HUANG HUAXUE
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a method for extracting high-purity neohesperidin from an immature bitter orange. The method comprises the following steps: (1) crushing and impregnating an immature bitter orange raw material in water, adding a compound plant-hydrolase preparation, and performing enzymatic decomposition to obtain an enzymatically decomposed immature bitter orange; (2) performing heating extraction, cooling, centrifugation and filtration on the enzymatically decomposed immature bitter orange to obtain enzymatic immature bitter orange extract; (3) performing water bath heat preservation on the enzymatic immature bitter orange extract, adding a flocculating agent for flocculation treatment, and performing centrifugation and filtration to obtain immature bitter orange flocculation liquid; (4) performing nanofiltration, concentration and vacuum drying on the immature bitter orange flocculation liquid to obtain a neohesperidin crude product; (5) completely dissolving the neohesperidin crude product in an et