Preparation method of Corbicula fluminea polypeptide chelated zinc

The invention discloses a preparation method of Corbicula fluminea polypeptide chelated zinc with a high chelating rate. The preparation method comprises the steps of: 1) preparing Corbicula fluminea homogenate; 2) adding neutral protease, and performing enzymolysis in constant temperature water bat...

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Bibliographische Detailangaben
Hauptverfasser: LIU JINGJING, ZHANG YING, CHENG RUNQING, DU YUXIN, ZHANG LEI, WANG XUEFENG, HAN YAOPING, DAI YANGJUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a preparation method of Corbicula fluminea polypeptide chelated zinc with a high chelating rate. The preparation method comprises the steps of: 1) preparing Corbicula fluminea homogenate; 2) adding neutral protease, and performing enzymolysis in constant temperature water bath; 3) conducting enzyme deactivation after enzymolysis; 4) carrying out centrifugal treatment to remove precipitate so as to obtain supernatant, i.e. Corbicula fluminea enzymolysis liquid; 5) subjecting the Corbicula fluminea enzymolysis liquid to vacuum freeze drying so as to obtain Corbicula fluminea polypeptide powder; 6) preparing a Corbicula fluminea polypeptide solution; 7) adding zinc vitriol and the Corbicula fluminea polypeptide solution (with a pH value of 5.06-6) to perform chelation; 8) carrying out two-stage chelation reaction; 9) then conducting chelation reaction; 10) removing the supernatant and collecting the precipitate; and 11) conducting vacuum freeze drying to obtain Corbicula fluminea polypept