Preparation method of Corbicula fluminea polypeptide chelated zinc
The invention discloses a preparation method of Corbicula fluminea polypeptide chelated zinc with a high chelating rate. The preparation method comprises the steps of: 1) preparing Corbicula fluminea homogenate; 2) adding neutral protease, and performing enzymolysis in constant temperature water bat...
Gespeichert in:
Hauptverfasser: | , , , , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses a preparation method of Corbicula fluminea polypeptide chelated zinc with a high chelating rate. The preparation method comprises the steps of: 1) preparing Corbicula fluminea homogenate; 2) adding neutral protease, and performing enzymolysis in constant temperature water bath; 3) conducting enzyme deactivation after enzymolysis; 4) carrying out centrifugal treatment to remove precipitate so as to obtain supernatant, i.e. Corbicula fluminea enzymolysis liquid; 5) subjecting the Corbicula fluminea enzymolysis liquid to vacuum freeze drying so as to obtain Corbicula fluminea polypeptide powder; 6) preparing a Corbicula fluminea polypeptide solution; 7) adding zinc vitriol and the Corbicula fluminea polypeptide solution (with a pH value of 5.06-6) to perform chelation; 8) carrying out two-stage chelation reaction; 9) then conducting chelation reaction; 10) removing the supernatant and collecting the precipitate; and 11) conducting vacuum freeze drying to obtain Corbicula fluminea polypept |
---|