Special culture medium for tissue culture of bamboo reeds and culture method

The invention provides a special culture medium for tissue culture of bamboo reeds. The special culture medium comprises the following components according to the content: an MS basic culture medium, 6g/l of agar, 0.5mg/l-1mg/l of indolebutyric acid (IBA), 0.1mg/l-0.5mg/l of active carbon and 0.5mg/...

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Bibliographische Detailangaben
Hauptverfasser: LIU MIN, LU JINYING, YANG SHUO, LI JING, LIU BAOPING, LIU CICI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a special culture medium for tissue culture of bamboo reeds. The special culture medium comprises the following components according to the content: an MS basic culture medium, 6g/l of agar, 0.5mg/l-1mg/l of indolebutyric acid (IBA), 0.1mg/l-0.5mg/l of active carbon and 0.5mg/l-1mg/l of thidiazuron. In a bamboo reed tissue culture propagation process, the low dosage of TDZ is added into the culture medium for the first time; and at a propagation culture phase, a lot of sprouts can be generated, and the propagation amount is 1.3-1.5 time as much as that of the culture medium without the TDZ. 本发明提供了种芦竹组织培养专用培养基,包含组分及含量如下:MS基本培养基、琼脂6g/l、吲哚丁酸(IBA)0.5-1mg/l、活性炭0.1-0.5mg/l、噻苯隆0.5-1mg/l。在芦竹组培繁殖过程中,本申请首次在培养基中添加了低剂量的TDZ,在增殖培养阶段,同时会产生大量的芽,增繁量为不添加TDZ的培养基时的1.3-1.5倍。