Special culture medium for tissue culture of bamboo reeds and culture method
The invention provides a special culture medium for tissue culture of bamboo reeds. The special culture medium comprises the following components according to the content: an MS basic culture medium, 6g/l of agar, 0.5mg/l-1mg/l of indolebutyric acid (IBA), 0.1mg/l-0.5mg/l of active carbon and 0.5mg/...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a special culture medium for tissue culture of bamboo reeds. The special culture medium comprises the following components according to the content: an MS basic culture medium, 6g/l of agar, 0.5mg/l-1mg/l of indolebutyric acid (IBA), 0.1mg/l-0.5mg/l of active carbon and 0.5mg/l-1mg/l of thidiazuron. In a bamboo reed tissue culture propagation process, the low dosage of TDZ is added into the culture medium for the first time; and at a propagation culture phase, a lot of sprouts can be generated, and the propagation amount is 1.3-1.5 time as much as that of the culture medium without the TDZ.
本发明提供了种芦竹组织培养专用培养基,包含组分及含量如下:MS基本培养基、琼脂6g/l、吲哚丁酸(IBA)0.5-1mg/l、活性炭0.1-0.5mg/l、噻苯隆0.5-1mg/l。在芦竹组培繁殖过程中,本申请首次在培养基中添加了低剂量的TDZ,在增殖培养阶段,同时会产生大量的芽,增繁量为不添加TDZ的培养基时的1.3-1.5倍。 |
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