Kit and method for carrying out typing detection on rickettsia by using multiplex real-time fluorescent quantitative polymerase chain reaction (PCR)

The invention discloses a nucleic acid group, a kit and a detection method for carrying out typing detection on rickettsia by using a real-time fluorescent quantitative polymerase chain reaction (PCR), and in particular relates to determination for Belli group rickettsia, spotted fever group rickettsiae and typhus rickettsiae. The kit provided by the invention is convenient to use, fewer in used reagents, low in cost, high in detection specificity and high in sensitivity; after the detection method is adopted, the rickettsia of the same sample is detected in a manner of genetic typing, so that the operation process is greatly simplified, the repeated operation steps are reduced, the time is shortened, the labor force consumed by repeated operation is reduced, and the cost is effectively lowered; therefore, the rapid screening for a rickettsia genotype is realized. 本发明公开了采用实时荧光定量PCR对立克次体分型的检测的核酸组、试剂盒及检测方法，具体用于对贝利群立克次体、斑点热群立克次体和斑疹伤寒立克次体的测定。本发明提供的试剂盒，使用方便，所用的试剂少，成本低，检测的特异性强，灵敏度高；检测方法实现了对同个样本，实现对立克次体的基因分型的检测，大大简化