Eimeria tenella microneme protein-2 mutant EtMIC2-2119 of chickens

The invention relates to the field of gene engineering and provides an eimeria tenella microneme protein-2 mutant EtMIC2-2119 of chickens. The mutant is obtained as follows: in-vitro orthogenesis is performed with EtMIC2 (GenBank:KC333870.1) of wild eimeria tenella SD-01 as basic protein, a gene mutation library is established, yeast is used for surface display of a protein mutant library, altered protein with immunogenicity and immune protection remarkably improved is screened by means of sorting by a flow cytometry, animal immune protection effect evaluation and the like, and the mutant is obtained finally. The mutant altered protein has 11 amino acid mutation sites; the immune protection effect ACI of altered EtMIC2 is improved by about 25.06% while compared with that of natural EtMIC2, and the mutant can serve as a vaccine to be applied in the field of resistance to infection by eimeria tenella of the chickens. 本发明涉及基因工程和遗传工程领域，提供了种鸡柔嫩艾美尔球虫微线蛋-2突变体EtMIC2-2119，该突变体是以野生型柔嫩艾美耳球虫SD-01的EtMIC2(GenBank:KC333870.