Preparation method of lactobacillus acidophilus feed additive

The invention relates to a preparation method of a lactobacillus acidophilus feed additive. The preparation method comprises the following steps of: carrying out size mixing on raw starch with buffer solution, so as to obtain fermenting mash; adding mixed enzyme of amylase and glucoamylase into the mash, reacting, centrifuging mash after reaction is finished, carrying out hot air drying on the obtained sediment, and smashing, so that mixture A is obtained; activating lactobacillus acidophilus, enlarging cultivation, inoculating into a fermentation liquid culture medium, and carrying out anaerobic culture for 18-20 hours, so that lactobacillus acidophilus culture solution is obtained; taking polygonatum kingianum, semen euryales and phaseolus calcaratus, carrying out superfine grinding, decocting, and concentrating, so that concentrated solution is obtained; adding the concentrated solution into the lactobacillus acidophilus culture solution, and continuously carrying out anaerobic culture for 3-4 hours, so th