Method for in-vitro oriented screening and verification of acetochlor resistant bodies for peanuts

The invention provides a method for in-vitro oriented screening and verification of acetochlor resistant bodies for peanuts. The method mainly comprises the steps that oriented screening for acetochlor resistant bodies is carried out on peanut explants cultured and obtained after induced mutation on a culture medium with acetochlor added; four weeks later, survival explants are transferred to a recovery growth culture medium to be cultured; regenerated plants are obtained through screening, leaves of the regenerated plants are taken and put into an acetochlor solution, and verification of acetochlor resistance is carried out. According to the method, acetochlor resistant bodies are screened and verified in an oriented mode through in-vitro culture, operation is convenient, limitation of seasons is broken through, and a great amount of manpower and a great quantity of material resources and financial resources can be saved. 本发明提供了种花生离体定向筛选和鉴定抗乙草胺体的方法，主要步骤是将诱变后培养获得的花生外植体在添加了乙草胺的培养基上，进行抗乙草胺体的定向筛选。4周后将存活的外植体转移到恢复