Protein detection method and protein detection kit

The invention relates to a novel protein detection method and a corresponding protein detection kit, in particular to an improved biuret colorimetric method. According to the improved biuret colorimetric method, an aqueous solution of sodium n-alkyl sulfate and sodium hydroxide serves as a reagent A, an aqueous solution of copper sulfate or copper sulfate hydrate, sodium tartrate and sodium hydroxide serves as a reagent B, and therefore the method can be suitable for rapidly and accurately determining the protein content in samples containing protein which is poor in solubility. Meanwhile, the protein detection kit has the advantages of being easy to prepare, low in cost, long in guarantee period and the like. 本发明涉及新的蛋白检测方法、以及相应的蛋白检测试剂盒。具体而言，本发明涉及改良的双缩脲比色法，通过采用直链烷基硫酸钠和氢氧化钠的水溶液作为试剂A，并采用硫酸铜或水合硫酸铜、酒石酸钠和氢氧化钠的水溶液作为试剂B，从而能够适用于对含有溶解性差的蛋白的样品中的蛋白含量进行快速、准确的测定。同时，本发明所述的蛋白检测试剂盒具有配制简单、成本低廉和保质期长等优点。