Liquid chip method for identifying pig origin food source bacteria

The invention relates to a liquid chip method for identifying pig origin food source bacteria such as Escherichia coli O157, non-O157 Escherichia coli, Shigella, salmonella, Yersinia enterocolitica and staphylococcus aureus. The method comprises the steps of extracting to-be-tested bacteria DNA as a template, using six pairs of primers shown in SEQ ID NO.1-12 for multi-PCR amplification, performing detecting on a Luminex 200 instrument after an amplified product is hybridized with microspheres, and determining a result according to the detection numerical value. According to the liquid chip method built by means of the synthesized primers, five different kinds of food source bacteria which can cause food poisoning can be detected out in a detection system, and only about 18-24 h is needed, so that the detection time is greatly shortened and manpower and material resources are saved compared with traditional animal food microbiological examination including isolated culture and biochemical serological tests ne