Single-component TMB (Tetramethylbenzidine) developing solution and preparation method thereof

The invention relates to a single-component TMB (Tetramethylbenzidine) developing solution and a preparation method thereof. The single-component TMB developing solution is prepared from the following components: TMB, dimethyl sulfoxide, glycerol, an oxidant, a sugar type compound or a derivative thereof and a buffering solution; the pH value of the single-component TMB developing solution is 3.0-7.0; the buffering solution is selected from a citric acid-sodium citrate buffering solution, a citric acid-EDTA (Ethylene Diamine Tetraacetic Acid) buffering solution, a citric acid-sodium acetate buffering solution or a citric acid-disodium hydrogen phosphate; and the sugar type compound is selected from at least one of glucoside or a derivative thereof, dextran or a derivative thereof and ficoll or a derivative thereof. The single-component TMB developing solution can be used for experiments including Western, IHC or in-situ hybridization and the like and is high in sensitivity, good in stability and long in prese