Primer, probe, composition and method for screening and identifying MLL rearrangement correlated fusion genes by utilizing multi-fluorescent polymerase chain reaction (PCR) technology

The invention relates to a primer, a probe, a composition and a method for screening and identifying MLL rearrangement correlated fusion genes by utilizing a multi-fluorescent polymerase chain reaction (PCR) technology. The screened MLL rearrangement correlated fusion genes comprise thirteen relatively common fusion genes, i.e., MLL-AF9, MLL-AF6, MLL-AF4, MLL-AF1P, MLL-AF1Q, MLL-AF10, MLL-AF17, MLL-AFX1, MLL-ELL, MLL-ENL, MLL-SEPT6, MLL-CBP and dup MLL. The primer, the probe, the testing combination way and the detection method are convenient, economical, fast, good in specificity, high in sensitivity and large in flux, thus being suitable for clinical test of large-batch samples. 本发明种用多重荧光PCR技术筛查MLL重排相关融合基因的引物、探针、组合物和方法。所述筛查的MLL重排相关融合基因包括：MLL-AF9、MLL-AF6、MLL-AF4、MLL-AF1P、MLL-AF1Q、MLL-AF10、MLL-AF17、MLL-AFX1、MLL-ELL、MLL-ENL、MLL-SEPT6、MLL-CBP、dupMLL共计13种相对常见融合基因。本发明所述引物、探针、检测组合方式和检测方法方便、经济、快捷，特异性好，灵敏度高，通量大，适于大批量样本的临床检测。