Method for isolating exosomes

The invention relates to a method for isolating exosomes from a biological fluid, said method including at least the following two consecutive steps of affinity purification: a) a first step using at least one specific anti-ligand of a generic exosome ligand, in order to obtain a population P of exosomes, said exosomes being separated from said anti-ligand; and b) a second step, applied to the population P of exosomes, using at least one anti-ligand specific to a ligand characteristic of a sub-population SP of exosomes, in order to obtain said sub-population SP of exosomes, said exosomes optionally being separated from said anti-ligand. The invention also relates to the uses of such a method. 本发明涉及种用于从生物液体中分离外泌体的方法所述方法包括至少下述两个连续的亲和纯化步骤:a)第步骤使用外泌体通用配体的至少种特异性抗配体，以便获得外泌体群体P，将所述外泌体与所述抗配体分离，和b)第二步骤，其应用于所述外泌体群体P，使用外泌体亚群体SP特有配体的至少种特异性抗配体，以便获得所述外泌体亚群体SP，将所述外泌体任选与所述抗配体分离。本发明还涉及此方法的应用。