Diagnostic kit for jointly detecting HIV antigen and HIV antibody and preparation method of diagnostic kit

The invention provides a diagnostic kit for jointly detecting an HIV antigen and an HIV antibody. The HIV-1type p24 antigen and the HIV-1/2 type antibody are qualitatively detected by adopting the immunoadsorption test principle of a double antibody sandwich method and a double antigen sandwich method and combining a time-resolved fluorescence immunoassay technique; the window period of existing HIV detection can be shortened to be 2-3 weeks, and the diagnostic kit is beneficial for early diagnosis of an HIV and also can serve as a basis for prognostically judging and evaluating the antiviral therapy effect; meanwhile, the kit further has the advantages of being high in specificity and sensitivity, good in repeatability, excellent in stability, wide in measurable range, high in detection automation degree, free of environmental pollution and the like. 本发明的种联合检测HIV抗原抗体的诊断试剂盒采用双抗体夹心法和双抗原夹心法的免疫吸附试验原理，结合时间分辨免疫荧光技术定性检测HIV-1型p24抗原和HIV-1/2型抗体；可以将目前HIV检测的窗口期缩短至2～3周，有助于HIV的早期诊断，还可以作为预后判断、评价抗病毒治疗效果的依据；同时本发明的试剂盒还具有特异性强、