Manufacturing method of epidermis for Tillandsia epidermis structure observation

The invention discloses a manufacturing method of epidermis for Tillandsia epidermis structure observation. The manufacturing method comprises 1, taking fresh mature leaves and immersing the leaves in a hydrogen peroxide solution at the room temperature, 2, taking out the leaves and scraping surface...

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Hauptverfasser: ZHOU DAOHONG, LI BING, GUAN BIN, BAO RONGJING, SHEN ZHENGHONG, TANG RONGDI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a manufacturing method of epidermis for Tillandsia epidermis structure observation. The manufacturing method comprises 1, taking fresh mature leaves and immersing the leaves in a hydrogen peroxide solution at the room temperature, 2, taking out the leaves and scraping surface exfoliate in the middles of the upper and lower surfaces, 3, cutting the leaf parts without exfoliate and tearing down the epidermis of the upper or lower surface, and 4, cleaning the epidermis by clear water, dipping the epidermis into water drops on a glass slide, unfolding the epidermis, covering the epidermis with cover glass and carrying out conventional dyeing. Compared with the prior art, the manufacturing method can take the whole epidermis, substantially improve an exfoliate removal rate and improve epidermis cell clarity and pore clarity, has simple processes and outstanding effects and is especially suitable for Tillandsia. 本发明公开了种用于气生铁兰属植物表皮结构观测的表皮制作方法,包括以下步骤:(1)取新鲜的成熟叶片,室温下浸泡在双氧水溶液中;(2)浸泡后取出叶片,在上表面和下表