Preparation and application for reaction type fluorescein fluorescent probe capable of detecting mercury ions
The invention provides preparation and application for a reaction type fluorescein fluorescent probe capable of detecting mercury ions. The preparation method for the reaction type fluorescein fluorescent probe capable of detecting the mercury ions comprises the following steps: dissolving fluoresce...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides preparation and application for a reaction type fluorescein fluorescent probe capable of detecting mercury ions. The preparation method for the reaction type fluorescein fluorescent probe capable of detecting the mercury ions comprises the following steps: dissolving fluorescein into a solvent so as to obtain a fluorescein solution, adding an acid binding agent, subjecting the obtained mixture to fully cooling, adding an acryloyl chloride solution into the above-mentioned mixed solution, carrying out a reaction at a room temperature, after the reaction is completed, carrying out rotary evaporation so as to remove the solvent, and carrying out drying so as to obtain an acryloyl fluorescein; and dissolving the acryloyl fluorescein into a solvent, dropwise adding a hydrazine hydrate solution, carrying out a reaction for 10 h to 14 h, subjecting the obtained product to recrystallization, and carrying out drying so as to obtain the reaction type fluorescein fluorescent probe capable of detec |
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