Method for improving screening efficiency of target traits in plant radiation mutation breeding

The invention discloses a method for improving screening efficiency of target traits in plant radiation mutation breeding. The method comprises the following steps: conducting radiation mutation treatment on plant seeds to obtain M0 seeds; subjecting the M0 seeds to multiple-sample coarse transplanting, collecting main spike seeds to obtain M1 seeds, conducting self seed-setting on M1 seeds to obtain M2 seeds; subjecting M2 seeds to seedling culture in indoor, growing the seedling to two leaves with one bud, and extracting genomic DNA from each individual plant. The characteristic of radiation mutation accelerating the mutation rate of an organism's genome is used; in the seedling stage, molecular biology technique is used for directed screening of target trait mutants from radiation mutation group, so as to reduce the work lead for gene without mutation or mutation unqualified for the target traits, and improve the accuracy of screening of target trait mutants. 本发明公开了种提高植物辐射诱变育种目标性状定向筛选效率的方法，包括如下步骤，对植物种子进行辐射