Primers for distinguishing riemerlla antipestifer from escherichia coli and distinguishing method

The invention provides primers for distinguishing riemerlla antipestifer from escherichia coli and a distinguishing method. Two pairs of specific primers are designed according to the conserved sequences of the riemerlla antipestifer outer membrane protein A (OmpA) gene and the escherichia coli glyceraldehyde-3-phosphate dehydrogenase A gene, the two ends of pig mitochondrial gene segments are labeled with the two pairs of specific primers respectively to form two different capture probes, and after the capture probes and to-be-detected bacteria are subjected to genomic hybridization, gap filling-in and cyclization, the capture probes are amplified reversely by using one pair of primers, wherein the amplification sizes of riemerlla antipestifer and escherichia coli are 504 bp and 328 bp respectively. So far there is no PCR method for distinguishing riemerlla antipestifer from escherichia coli by using only one pair of primers. 本发明提供了种区别鸭疫里默氏菌和大肠杆菌的引物及其方法，根据鸭疫里默氏菌外膜蛋白A基因和大肠杆菌glyceraldehyde-3-phosphate dehydrog