HIV (human immunodeficiency virus) inhibitor screening model and preparation method and application thereof

The invention relates to an HIV (human immunodeficiency virus) inhibitor screening model. The HIV screening model consists of a cell model I, a cell model II and a cell model III, wherein the cell model I is an HEK293T cell which is transfected by recombinant plasmids gp120/pEGFP-N3 and CD4/pDsRed-N...

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Bibliographische Detailangaben
Hauptverfasser:	Li Baihua, Ma Weifeng, Duan Siqin, Fan Yao, Zhao Xue, Li Yujing
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an HIV (human immunodeficiency virus) inhibitor screening model. The HIV screening model consists of a cell model I, a cell model II and a cell model III, wherein the cell model I is an HEK293T cell which is transfected by recombinant plasmids gp120/pEGFP-N3 and CD4/pDsRed-N1; the cell model II is an HEK293T cell which is transfected by recombinant plasmids gp120/pDsRed-N1 and CXCR4/pEGFP-N3; the cell model III is an HEK293T cell which is transfected by recombinant plasmids CD4/pEGFP-N3 and CXCR4/pDsRed-N1; the sequence of a gene gp120 which is inserted into the recombinant plasmids gp120/pDsRed-N1 and gp120/pEGFP-N3 is shown in SEQ ID NO.1; the sequence of a gene CD4 which is inserted into the recombinant plasmids CXCR4/pEGFP-N3 and CXCR4/pDsRed-N1 is shown in SEQ ID NO.2; the sequence of a gene CXCR4 which is inserted into the recombinant plasmids CXCR4/pEGFP-N3 and CXCR4/pDsRed-N1 is shown in SEQ ID NO.3. The HIV screening model has the advantages that the feature of FRET (fluoresc