Nanometer chitin-lysozyme composite particles and preparation method thereof

The invention relates to a preparation method of nanometer chitin-lysozyme composite particles. The method comprises the steps that 1, chitin is dissolved in 2.5-3 M HCl to prepare a solution with the mass-volume concentration being 30-35% is prepared; intense stirring is carried out for 1.5-6 h at 95-105 DEG C for acid hydrolysis; turbid liquid obtained after hydrolysis is centrifuged, washed with water and precipitated; the precipitate obtained after washing is dispersed in water to obtain turbid liquid again, and the obtained turbid liquid is placed in a dialysis bag for dialysis; a sample obtained after dialysis is subjected to ultrasonic treatment for 2-5 min and then placed under the temperature of -85-75 DEG C to be frozen, vacuum freeze drying is carried out, and nanometer chitin is obtained; 2, the nanometer chitin is dissolved in a phosphate buffer solution to prepare a solution with the concentration of 1.96*10-9.84*10 M, and the solution is subjected to ultrasonic treatment for 1-2 min to