Molecular marker closely linked with melon pseudoperonospora cubensis resisting gene and application thereof
The invention discloses a molecular marker closely linked with a melon pseudoperonospora cubensis resisting gene and the application thereof. Based on the seedling-stage resource pseudoperonospora cubensis resistance identification and selection principle and the prior art, a melon resource PI438685...
Gespeichert in:
Hauptverfasser: | , , , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses a molecular marker closely linked with a melon pseudoperonospora cubensis resisting gene and the application thereof. Based on the seedling-stage resource pseudoperonospora cubensis resistance identification and selection principle and the prior art, a melon resource PI438685 with high pseudoperonospora cubensis resistance is selected as the high-resistance resource, a melon pseudoperonospora cubensis anti-fever F2-generation group is established by means of the high-resistance resource PI438685 and the susceptible farm variety Clarke, the molecular marker SSR-666230 linked with the PI438685 pseudoperonospora cubensis resisting gene is found by means of well-known heredity software, and the genetic distance between the marker and the resistance gene is 2.66 cm. By extracting genome DNA of a single melon plant and conducting PCR amplification with the marker SSR-666230, whether a unique 230bp specific band appears is detected, it is predicted that the single melon plant has pseudoperono |
---|