Optimal extraction technology of infectious bursal diseased egg yolk immunoglobulin

The invention discloses an optimal extraction technology of infectious bursal diseased egg yolk immunoglobulin. The technology comprises the following steps: Step 1, obtaining a highly immunized egg yolk antibody: firstly carrying out subcutaneous injection of multiple immunizing doses of chicken bursal inactivated oil-emulsion vaccines to obtain basic immunity, enhancing immunity and strengthening immunity and maintaining; Step 2, extracting egg yolk; Step 3, degreasing and layering the egg yolk immunoglobulin; and Step 4, extracting an egg yolk antibody. After layering hens are immunized for many times, antibody level is raised. Antibody level titer of infectious bursal diseased egg yolk reaches 10lg2 and above. Test results show that antibody level rises slowly within 1-5 days after immunization and rises rapidly within 10-15 days after immunization. 本发明公开了种传染性法式囊卵黄免疫球蛋白优化提取工艺，包括如下步骤，第1步是获取高免卵黄抗体：首先用多个免疫剂量的鸡法氏囊油乳剂灭活苗皮下注射获取基础免疫，其次加强免疫，然后再强化免疫并维持。第2步是提取卵黄液。第3步是卵黄免疫球蛋白去脂分层。第4步是提取卵黄抗体。本发明中将蛋鸡经多次免疫后，抗体水平提高。鸡蛋传染