Recombinant eukaryotic bacterial strain for producing taxadiene and method for preparing taxadiene by using recombinant eukaryotic bacterial strain

The invention belongs to the field of bioengineering technology, and discloses a recombinant eukaryotic bacterial strain for producing taxadiene and a method for preparing taxadiene by using the recombinant eukaryotic bacterial strain. A gene expression cassette for expressing GGPP synthase A, a gen...

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Bibliographische Detailangaben
Hauptverfasser: ZHAI FANG, YUAN YINGJIN, SONG TIANQING, LI BINGZHI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of bioengineering technology, and discloses a recombinant eukaryotic bacterial strain for producing taxadiene and a method for preparing taxadiene by using the recombinant eukaryotic bacterial strain. A gene expression cassette for expressing GGPP synthase A, a gene expression cassette for expressing taxadiene synthase, a gene expression cassette for expressing 3-hydroxy-3-methylglutaryle-CoA reductase, and a fusion gene expression cassette containing a gene for expressing FPP synthase and a gene for expressing GGPP synthase B are introduced into the eukaryotic bacterial strain in order to form the recombinant eukaryotic bacterial strain; a promoter of the gene expression cassette for expressing GGPP synthase A is TDH1; a promoter of the gene expression cassette for expressing taxadiene synthase is ENO2; a promoter of the gene expression cassette for expressing 3-hydroxy-3-methylglutaryle-CoA reductase is PDC1; and a promoter of the fusion gene expression cassette is GPM1. T