Siphoninus phillyreae (Haliday) specific SS-COI primers as well as rapid PCR detection method and kit

The invention relates to the field of molecular biology, in particular to a siphoninus phillyreae (Haliday) specific SS-COI primer as well as a rapid PCR detection method and kit. The primer sequences are primer SPZWCF1: 5'-AAGAGAATTAAGTAATAGAGGG-3' and primer SPZWCR1: 5'-GATTCAAATCTT...

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Hauptverfasser:	XIAN XIAOQING, GUO JIANYING, ZHANG GUIFEN, WANG YUSHENG, SHAO JIAWEN, WAN FANGHAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of molecular biology, in particular to a siphoninus phillyreae (Haliday) specific SS-COI primer as well as a rapid PCR detection method and kit. The primer sequences are primer SPZWCF1: 5'-AAGAGAATTAAGTAATAGAGGG-3' and primer SPZWCR1: 5'-GATTCAAATCTTATACCCAATAGT-3'. Specific primers of the invention not only can detect single adult siphoninus phillyreae (Haliday), but also can accurately detect single eggs, newly hatched nymphs, second instar nymphs, third instar nymphs, fourth instar nymphs, subnymphs and the like. 本发明涉及分子生物学领域，具体地，本发明梣粉虱特异性SS?COI引物及快速PCR检测方法和试剂盒。所述引物序列为：引物SPZWCF1：5′?AAGAGAATTAAGTAATAGAGGG?3′；和引物SPZWCR1：5′?GATTCAAATCTTATACCCAATAGT?3′。本发明的特异性引物不仅对梣粉虱的单头成虫可以进行检测，对于单粒卵和初孵若虫、2龄若虫、3龄若虫、4龄若虫、拟蛹等也能准确检测。