Thermophilic beta-1, 4-xylanase-His fusion protein, preparation method and application, and construction of genetic engineering bacteria thereof

The invention provides a construction method of thermophilic beta-1, 4-xylanase-His fusion protein genetic engineering bacteria. The nucleotide sequence of the thermophilic beta-1, 4-xylanase-His fusion protein gene is shown as SEQ ID NO.1, and the construction method comprises the steps of: step A: cultivation of Geobacillus thermoleovorans; step B: genomic DNA extraction and fusion protein gene sequence construction; step C: primer design and fishing of a thermophilic beta-1, 4-xylanase-His fusion protein target gene by polymerase chain reaction (PCR); step D: construction of a recombinant expression vector; and step E: transformation of the recombinant vector into a host cell. The thermophilic beta-1, 4-xylanase involved in the invention has very high thermostability, alkaline environment resistance and resistance to metal ions, organic solvents and surfactants, and can be used in biomass energy production, functional sugar, and functional sugar alcohol production. 本发明提供了种嗜热β-1，4木聚糖酶-His融合蛋白基因工程菌的构建方法，所述的嗜