Method for improving unit mass solid medium fermentation efficiency of ophiocordyceps sinensis

The invention relates to a method for improving the unit mass solid medium fermentation efficiency of ophiocordyceps sinensis. The method comprises a formula of a solid medium, a preparation method and an inoculation method, wherein the formula of the solid culture medium comprises 1-5g of peptone,...

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Hauptverfasser: LIU XIN, ZHONG XIN, SHEN DONGXIU, WANG HAIZHEN, LI SHAOSONG, KAN XUTIAN, ZHANG GUREN, GU LI
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creator LIU XIN
ZHONG XIN
SHEN DONGXIU
WANG HAIZHEN
LI SHAOSONG
KAN XUTIAN
ZHANG GUREN
GU LI
description The invention relates to a method for improving the unit mass solid medium fermentation efficiency of ophiocordyceps sinensis. The method comprises a formula of a solid medium, a preparation method and an inoculation method, wherein the formula of the solid culture medium comprises 1-5g of peptone, 0.5-2.5g of yeast extract, 6-15g of egg homogenate, 3-5g of trehalose, 0.5-2g of glucose, 0.5-1g of lignin, 0.4-1g of palmitic acid, 0.8-2g of oleic acid, 0.2-0.6g of linoleic acid or 1.5-4g of mixed liquid of peanut oil or corn oil and olive oil in every 100mL of water; the final thickness of the solid medium is 1-3cm in preparation; and in inoculation, a liquid fermentation strain liquid is adopted according to a ratio of 0.2-0.8mL per centimeter. By adopting the method provided by the invention, growth of ophiocordyceps sinensis can be effectively promoted, the mycelia content generated from the solid medium by a unit mass can be increased, and the fermentation production efficiency of ophiocordyceps sinensis ca
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Method for improving unit mass solid medium fermentation efficiency of ophiocordyceps sinensis
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