Method for preparing total flavonoids from eucommia male flowers

The invention discloses a method for preparing total flavonoids from eucommia male flowers. The method comprises the following steps: grinding the eucommia male flowers, and sieving with a 40-mesh sieve; adding to ethanol, extracting the sieved eucommia male flowers twice for 2 to 3 hours at the temperature of 50 to 90 DEG C, and combining filtrates to obtain mixed liquid; concentrating the mixed liquid, adding the concentrated mixed liquid to clear water, carrying out water precipitation for 0.5 to 1.5 hours, filtering to obtain a second solid, adding the second solid to an ethanol solution, and filtering insoluble matters to obtain a crude extract of the total flavonoids; using a wet method to pack columns, placing an HPD-417 type macroporous adsorption resin into a chromatographic column to obtain a resin column, adding the crude extract of the total flavonoids obtained in the step 3 to the resin column, collecting an elution solution, concentrating and carrying out vacuum freeze drying to obtain purified