Reagent kit for detecting and typing high-risk type human papilloma viruses and application of reagent kit

The invention relates to a reagent kit for detecting and typing high-risk type human papilloma viruses and application of the reagent kit, and discloses a detection reagent particularly applicable to diagnosing the high-risk type human papilloma viruses (PHV) and typing the human papilloma viruses HPV16 and PHV18.The reagent kit and the method have the advantages that the detection reagent is obtained by means of reasonable design and optimization, is excellent in specificity and high in amplification efficiency and sensitivity when used for PCR (polymerase chain reaction) amplification, diversified high-risk type viruses can be simultaneously detected by the detection reagent, and detection programs are simple and are easy to implement; PCR amplification reaction programs are further optimized by an inventor, and accordingly the amplification efficiency further can be improved. 本发明涉及种高危型人乳头瘤病毒的检测和分型试剂盒及其应用。揭示了类特别适合于诊断高危型人乳头瘤病毒(HPV)以及对HPV16和HPV18进行分型的检测试剂，所述检测试剂经过合理的设计、优选而获得，用于PCR扩增时特异性良好、扩增效率高、灵敏度高，且可以同时检测多种