Molecular detection method for resistance of chilo suppressalis to chlorantraniliprole and primers special for method

The invention discloses a molecular detection method for resistance of chilo suppressalis to chlorantraniliprole and primers special for the method.The invention provides a specific primer composition which is composed of single-chain DNA molecules as shown in a sequence 1, a sequence 2 and a sequence 3 in a sequence table.The specific primer composition can be used for detecting whether an action site ryanodine receptor of chlorantraniliprole is subjected to gene G4946E mutation or not, G4946E mutation of a ryanodine receptor gene of chilo suppressalis means that the 14837 nucleotide located at the 5' tail end of an open reading frame of the ryanodine receptor gene is mutated into A from G, and therefore the resistance of chilo suppressalis to chlorantraniliprole is detected.The specific primer composition and application thereof have important significance in monitoring a development trend of the resistance of chilo suppressalis field populations to chlorantraniliprole, guiding reasonable pesticide use, del