Method of measuring purity of recombinant human calmodulin phosphatase B subunit

The invention relates to the field of protein purification, in particular to a method of measuring purity of a recombinant human calmodulin phosphatase B subunit.The method includes that theoretical plate number of a chromatographic column calculated through rhCNB dipolymer peak is greater than 5000, and tailing factor of the rhCNB dipolymer peak T is less than 2.0; a standard solution is continuously sampled for five times, and RSD of main peak area of the standard solution is less than 2.0%.Specificity verification results and evaluation show that separation degree of citric acid as well as citrate and rhCNB dipolymer is greater than 1.5; products of a strong destroying experiment include monomer and dipolymer, the rhCNB dipolymer can be separated from the monomer effectively, and the separation degree is greater than 1.5.High-temperature degradation and photodegradation products generated in the strong destroying experiment have no interference on measuring of the rhCNB dipolymer, and it shows that the sep