Method of preparing high-purity diphtheria toxin original liquid

The invention discloses a method of preparing high-purity diphtheria toxin original liquid, comprising eleven steps: (1), culturing viable bacteria, (2), deactivating formaldehyde, (3), separating a bacterial liquid, (4), removing bacteria and filtering, (5), ultra-filtering and concentrating, (6), ultra-filtering and dialyzing, (7), decoloring by ion exchange, (8), salting out, (9), desalting, (10), detoxifying the formaldehyde, and (11), removing bacteria and filtering.In the preparation process of the method, the technical scheme where an ion exchange unit is used to decolor articles is employed, the problems and effects that the prior art is long in preparation cycle, low in efficiency, poor in uniformity, difficult for purity improvement are overcome, and the preparation of the diphtheria toxin original liquid is imparted shortened preparation cycle, improved efficiency, improved quality uniformity and improved purity. 本发明公开了种高纯度白喉类毒素原液的制备方法，该方法包括活菌培养(1)、甲醛灭活(2)、菌液分离(3)、除菌过滤(4)、超滤浓缩(5)、超滤透析(6)、离子交换脱色(7)、