Method for extracting genomic DNA (deoxyribonucleic acid) from whole blood on basis of improved immunomagnetic bead method

The invention relates to a method for extracting genomic DNA (deoxyribonucleic acid) from whole blood on basis of an improved immunomagnetic bead method.The method includes steps: erythrocyte splitting, leukocyte splitting, DNA adsorption, DNA purification and DNA elution.DNA concentration and purity are measured by a DNA quantometer, sequencing of PCR (polymerase chain reaction) amplification products is realized by a sequencer, and HLA (human leukocyte antigen) classification result analysis is performed through relevant analysis software.The method has the advantages of high DNA concentration uniformity, high purity, substantial increase of HLA classification result success rate, and high acceptability of a signal-to-noise ratio, thereby being widely applicable to extraction of the genomic DNA from whole blood samples. 本发明涉及种基于改良磁珠法快速提取全血中基因组DNA的方法。该方法包括以下步骤：红细胞裂解、白细胞裂解及DNA吸附、DNA纯化和DNA洗脱。利用DNA定量仪测定DNA浓度与纯度，利用测序仪对PCR扩增产物进行测序，相关的分析软件进行HLA分型结果分析。本发明具有DNA浓度均性强、纯度高、HLA分型结果成功率大幅度提高和信噪比合格率高的特点。因此，可广泛地应用于全血样本中基因组D