Removal of residual cell culture impurities

The present application discloses methods for removing residual impurities from protein preparations. Such methods include addition of an anionic detergent to a solution comprising proteins of interest and cellular contaminants under non-precipitating conditions and passing the solution through an ion exchange column. 本发明公开了用于从蛋白质制备物中去除残留杂质的方法。这类方法包括在非沉淀条件下向包含感兴趣的蛋白质和细胞污染物的溶液中加入阴离子去污剂并将该溶液通过离子交换柱。