Human urine HIV1/2 antibody detection test paper through colloidal gold chromatographic method and preparation method thereof

The invention belongs to the field of medical immunologic application and discloses a human urine HIV1/2 antibody detection test paper through a colloidal gold chromatographic method and a preparation method thereof. The test paper is a test paper strip which is formed by successively laminating and...

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Hauptverfasser: YAN HAIJUN, GAO FEI, PIAO CHENGXI, LI JUNYE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of medical immunologic application and discloses a human urine HIV1/2 antibody detection test paper through a colloidal gold chromatographic method and a preparation method thereof. The test paper is a test paper strip which is formed by successively laminating and pasting a sample pad, a gold-coated polyester film, a coated nitrocellulose membrane and a water-absorbing pad surface on a substrate board, wherein a detection line and a quality control line are formed on the coated nitrocellulose membrane. In a detection zone of the nitrocellulose membrane, an HIV-1 gp41 antigen and an HIV-2 gp36 antigen are coated, and in the quality control zone, a goat-anti-human IgG polyantibody is coated. During detection, 200 [mu]l of urine from scrub acceptor by means of a 200 [mu]l transfer pipette, and the test paper strip is inserted into a test tube to detect the urine. In the invention, the specific antigens, HIV-2 gp36 and HIV-1 gp41, of AIDS are introduced into the HIV1/2 antibody