Cell detection methods and reagents having a releasable labelling moiety

The invention is directed to a method for detecting a target moiety in a sample of biological specimens by: a) providing at least one conjugate with the general formula (I) Xn-P-Ym , with X is an detection moiety, P an enzymatically degradable spacer and Y an antigen recognizing moiety and n, m are integers between 1 and 100 and wherein X and Y are covalently bound to P, b) contacting the sample of biological specimens with at least one conjugate, thereby labeling the target moiety recognized by the antigen recognizing moiety Y c) detecting the target moiety labeled with the conjugate with the detecting moiety X and d) enzymatically cleaving the covalent bonded detection moiety X from spacer P. The method is useful to identify target moieties on the biological specimens. The biological specimens detected by the conjugate can be subsequently removed from the sample. 本发明涉及检测生物样本的样品中的靶部分的方法，其包括：a)提供至少种通式(I)X-P-Y的缀合物，其中X是检测部分，P是可酶法降解的间隔物且Y是抗原识别部分，且n、m是1至100之间的整数，且其中X和Y与P共价键合，b)使生物样本的样品与至少种缀合物接触，由此标记被抗原识别部分Y识别的靶部分