Porcine pseudorabies virus positive serum preparation method

The invention discloses a porcine pseudorabies virus positive serum preparation method. A water-in-oil type inactivated vaccine is prepared through emulsification, and then porcine pseudorabies virus positive serum with a high neutralizing titer is obtained through immune reaction generated by stimulating sheep through the synergistic effect of a porcine pseudorabies virus live vaccine and the inactivated vaccine. The method reduces the risk of exogenous virus contamination caused by homologous animals when positive serum is prepared from pigs, and a new thought is provide for preparation of positive serum. 本发明公开了一种猪伪狂犬病病毒阳性血清的制备方法，其通过乳化制备油包水型灭活疫苗，然后通过猪伪狂犬病病毒活疫苗和灭活苗协同作用刺激羊体产生的免疫反应获得高中和效价的猪伪狂犬病病毒阳性血清。本发明减小了用猪制备阳性血清因同源动物而引起的同源其他外源病毒污染的风险，为阳性血清的制备提供了新的思路。