Cobra venom nerve growth factor purification and preparation method

The invention discloses a cobra venom nerve growth factor purification and preparation method. Firstly, a DEAE CL-6B anion exchange column is adopted and then a Sephadex G-50 gel chromatography column, a Macro-prep High S cation exchange column is utilized for separation in sequence, a PC12 cell test method is adopted to dilute collected peaks with ultra-pure water after each time of separation, and then testing is performed to select the peaks having the highest cobra venom nerve growth factor activity as samples for subsequent separation, wherein the minimum concentration of NGF induced PC12 cell differentiation is determined through dilution. An inventor finds peaks having the highest NGF activity in the cobra venom separation process and determines that the minimum concentration of the NGF induced PC12 cell differentiation is up to 0.1 micrograms/ml. Tests show that electrophoresed pure NGF can be obtained by applying the method and have high purity and high activity. 本发明公开了一种眼镜蛇毒神经生长因子纯化制备方法，先采用DEAE CL?6