Preparation method of porcine circovirus virus type 2 hyperimmune egg yolk antibody

The present invention discloses a preparation method of a porcine circovirus virus type 2 hyperimmune egg yolk antibody, first, a vaccine is prepared, to be more specific, surface of sterilely-acquired lungs, livers, brains and lymph node tissues of infected pigs is washed with a l000U / mL double-a...

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Hauptverfasser: JIANG QINGDONG, SUO JIANGHUA, HE JINHUAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The present invention discloses a preparation method of a porcine circovirus virus type 2 hyperimmune egg yolk antibody, first, a vaccine is prepared, to be more specific, surface of sterilely-acquired lungs, livers, brains and lymph node tissues of infected pigs is washed with a l000U / mL double-antibody liquid, 20% aluminum plastic saline solution is added in the ratio of 1: 2, and then the vaccine is prepared by processing; and the porcine circovirus virus type 2 self-tissue inactivated vaccine is intramuscularly injected into a laying hen to prepare the hyperimmune egg yolk antibody. The porcine circovirus virus type 2 self-tissue inactivated vaccine is used for immunizing chicken, a hyperimmune egg is collected to prepare the egg yolk antibody, the egg yolk antibody is used for treatment of the infected pigs, and by clinical validation, the porcine circovirus virus type 2 hyperimmune egg yolk antibody has good effect. 本发明公开了一种猪圆环病毒2型高免卵黄抗体的制备方法,首先自制疫苗,将无菌采集发病猪的肺脏、肝脏和脑及淋巴结组织,用l000U/mL的双抗液冲洗表面后按1:2的比例加入20