Duck hepatitis bivalent live vaccines and preparation method thereof

The invention relates to duck hepatitis bivalent live vaccines and a preparation method thereof. According to the invention, 1) SPF chicken embryos are inoculated with vaccine strains of the duck hepatitis bivalent live vaccines, namely DHV-1 QL1 strains, through allantoic cavities for culture, and are inoculated with DHV-3 QL3 strains through chorioallantoic membranes or yolk sacs for culture, the antigen contents of the prepared vaccines are high, and all not less than 10ELD50/ml, so that the duck hepatitis bivalent live vaccines have the characteristics of good safety and excellent immunogenicity, and break through limitations of DHV-3 type virus strains which only can be proliferated in duck embryos; 2) virus solutions cultured by the SPF chicken embryos lower risks of exogenous pathogenic microbial contamination, improve labor efficiency and reduce production costs, thereby being suitable for mass production; 3) the bivalent live vaccines disclosed by the invention can prevent DHV-1 type and DHV-3 t