Lipoprotein phospholipase A2 detection kit

The invention provides a lipoprotein phospholipase A2 detection kit which comprises reagent R1 and reagent R2, the reagent comprises a buffer solution, a stabilizing agent, electrolyte, a surface active agent and preservative; the reagent R2 comprises polystyrene latex particles coupled to an antibo...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: SHANG YIWEN, SHI CHUNLIN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a lipoprotein phospholipase A2 detection kit which comprises reagent R1 and reagent R2, the reagent comprises a buffer solution, a stabilizing agent, electrolyte, a surface active agent and preservative; the reagent R2 comprises polystyrene latex particles coupled to an antibody resisting to the lipoprotein phospholipase A2, a buffer solution, a stabilizing agent, a surface active agent and preservative. A directional coupling method is adopted for the polystyrene latex particles sensitized by the antibody resisting to the lipoprotein phospholipase A2, and the preparation steps include activation of the polystyrene latex particles; oxidation of the antibody resisting to the lipoprotein phospholipase A2; coupling of the antibody resisting to the lipoprotein phospholipase A2 to the polystyrene latex particles. The kit is high in detection sensitivity, wide in detection range, good in accuracy and high in interference resistance. 本发明提供了一种脂蛋白磷脂酶A2检测试剂盒,该试剂盒包括试剂R1和试剂R2,试剂R1包括缓冲液、稳定剂、电解质、表面活性