Method for performing germplasm identification on kernel-using apricots by SSR (simple sequence repeat) molecular markers
The invention relates to a method for performing germplasm identification on kernel-using apricots through combination of specific SSR (simple sequence repeat) molecular markers with a fluorescent capillary electrophoresis detection technology. The method comprises steps as follows: PCR (polymerase...
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| creator | WUYUNTA'NA BAO WENQUAN ZHAO HAN ZHU GAOPU |
| description | The invention relates to a method for performing germplasm identification on kernel-using apricots through combination of specific SSR (simple sequence repeat) molecular markers with a fluorescent capillary electrophoresis detection technology. The method comprises steps as follows: PCR (polymerase chain reaction) amplification is performed on standard germplasm by molecular marker primers; PCR amplification is performed on target germplasm by the molecular marker primers, and the target germplasm is to-be-identified germplasm; amplification results obtained from the two steps are detected with the fluorescent capillary electrophoresis detection technology and then are compared, germplasm identification is performed according to consistency or non-consistency of the comparison result, wherein the molecular markers are selected from following SSR markers: UDP98-409, UDP98-406, UDP97-401, pchgms12, MA039a, MA020a, BPPCT002, BPPCT007 and BPPCT008, and forward primer sequences and reverse primer sequences of the |
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The method comprises steps as follows: PCR (polymerase chain reaction) amplification is performed on standard germplasm by molecular marker primers; PCR amplification is performed on target germplasm by the molecular marker primers, and the target germplasm is to-be-identified germplasm; amplification results obtained from the two steps are detected with the fluorescent capillary electrophoresis detection technology and then are compared, germplasm identification is performed according to consistency or non-consistency of the comparison result, wherein the molecular markers are selected from following SSR markers: UDP98-409, UDP98-406, UDP97-401, pchgms12, MA039a, MA020a, BPPCT002, BPPCT007 and BPPCT008, and forward primer sequences and reverse primer sequences of the</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160629&DB=EPODOC&CC=CN&NR=105713981A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160629&DB=EPODOC&CC=CN&NR=105713981A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WUYUNTA'NA</creatorcontrib><creatorcontrib>BAO WENQUAN</creatorcontrib><creatorcontrib>ZHAO HAN</creatorcontrib><creatorcontrib>ZHU GAOPU</creatorcontrib><title>Method for performing germplasm identification on kernel-using apricots by SSR (simple sequence repeat) molecular markers</title><description>The invention relates to a method for performing germplasm identification on kernel-using apricots through combination of specific SSR (simple sequence repeat) molecular markers with a fluorescent capillary electrophoresis detection technology. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Method for performing germplasm identification on kernel-using apricots by SSR (simple sequence repeat) molecular markers |
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