Method for performing germplasm identification on kernel-using apricots by SSR (simple sequence repeat) molecular markers

The invention relates to a method for performing germplasm identification on kernel-using apricots through combination of specific SSR (simple sequence repeat) molecular markers with a fluorescent capillary electrophoresis detection technology. The method comprises steps as follows: PCR (polymerase...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: WUYUNTA'NA, BAO WENQUAN, ZHAO HAN, ZHU GAOPU
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention relates to a method for performing germplasm identification on kernel-using apricots through combination of specific SSR (simple sequence repeat) molecular markers with a fluorescent capillary electrophoresis detection technology. The method comprises steps as follows: PCR (polymerase chain reaction) amplification is performed on standard germplasm by molecular marker primers; PCR amplification is performed on target germplasm by the molecular marker primers, and the target germplasm is to-be-identified germplasm; amplification results obtained from the two steps are detected with the fluorescent capillary electrophoresis detection technology and then are compared, germplasm identification is performed according to consistency or non-consistency of the comparison result, wherein the molecular markers are selected from following SSR markers: UDP98-409, UDP98-406, UDP97-401, pchgms12, MA039a, MA020a, BPPCT002, BPPCT007 and BPPCT008, and forward primer sequences and reverse primer sequences of the