Method for performing germplasm identification on kernel-using apricots by SSR (simple sequence repeat) molecular markers
The invention relates to a method for performing germplasm identification on kernel-using apricots through combination of specific SSR (simple sequence repeat) molecular markers with a fluorescent capillary electrophoresis detection technology. The method comprises steps as follows: PCR (polymerase...
Gespeichert in:
Hauptverfasser: | , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention relates to a method for performing germplasm identification on kernel-using apricots through combination of specific SSR (simple sequence repeat) molecular markers with a fluorescent capillary electrophoresis detection technology. The method comprises steps as follows: PCR (polymerase chain reaction) amplification is performed on standard germplasm by molecular marker primers; PCR amplification is performed on target germplasm by the molecular marker primers, and the target germplasm is to-be-identified germplasm; amplification results obtained from the two steps are detected with the fluorescent capillary electrophoresis detection technology and then are compared, germplasm identification is performed according to consistency or non-consistency of the comparison result, wherein the molecular markers are selected from following SSR markers: UDP98-409, UDP98-406, UDP97-401, pchgms12, MA039a, MA020a, BPPCT002, BPPCT007 and BPPCT008, and forward primer sequences and reverse primer sequences of the |
---|