Small-interfering RNA, short hairpin RNA and carrier for mammal R-Spondin1 gene target as well as application thereof

The invention discloses a small-interfering RNA, a short hairpin RNA and a carrier for a mammal R-Spondin1 gene target as well as application thereof. The small-interfering RNA contains a positive-sense strand and an antisense strand. The short hairpin RNA is synthetized on the basis of the small-interfering RNA by virtue of a chemical synthesis method, and can be further connected with a virus expression vector or a non-virus expression vector so as to form the carrier for the mammal R-Spondin1 gene target; the virus expression vector is a lentiviral vector or an adenovirus vector, the non-virus expression vector is a plasmid vector; the carrier of the short hairpin RNA can be used for preparing gene therapy medicine for hepatic fibrosis. The RNA interference fragment designed for the R-Spondin1 gene target is capable of promoting static state back-formation or apoptosis of activated hepatic stellate cells and thus effectively promoting a hepatic fibrosis recovery process. 本发明公开了针对哺乳动物R-Spondin1基因靶点的小干扰RNA、短