Hybridoma cell strain 4C9 and anti-His tag protein monoclonal antibody produced by same

The invention discloses a hybridoma cell strain 4C9 and an anti-His tag protein monoclonal antibody produced by the same. The hybridoma cell strain 4C9 is collected in CCTCC with the collection number of CCTCC NO. C2015198, and can be used for preparing high-potency anti-His tag protein monoclonal antibody, the potency of anti-His tag protein mouse hydroperitoneum antibody adsorbed and measured by an ELISA (Enzyme Linked Immunosorbent Assay) method is up to 106; the anti-His tag protein monoclonal antibody provided by the invention is high in sensitivity and good in specificity; expression analysis on His tag protein is accurate, the hybridoma cell strain 4C9 can be applied to measurement and identification on expression of recombinant protein with His tag protein and measurement on concentration of recombinant protein with His tag. 本发明公开了种杂交瘤细胞株4C9及其产生的抗His标签蛋白单克隆抗体，该杂交瘤细胞株4C9保藏在中国典型培养物保藏中心，保藏号为：CCTCC NO. C2015198，其可以用于制备高效价抗His标签蛋白单克隆抗体，抗His标签蛋白鼠腹水抗体酶联免疫吸附分析（ELISA）法测得的效价可达10；本发明提供的抗His标签蛋白单克隆抗体灵敏度高、特异性好，对Hi