A method of extracting volatile oil from armillaria luteo-virens

A method of extracting volatile oil from armillaria luteo-virens is disclosed. The method includes subjecting a fruiting body of the armillaria luteo-virens to superfine grinding, adding acetone, performing cold leaching and extracting, filtering to obtain a filtrate, centrifuging the filtrate to ob...

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Bibliographische Detailangaben
Hauptverfasser: TANG CHUCHEN, ZHANG YAOZHOU, MA LIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:A method of extracting volatile oil from armillaria luteo-virens is disclosed. The method includes subjecting a fruiting body of the armillaria luteo-virens to superfine grinding, adding acetone, performing cold leaching and extracting, filtering to obtain a filtrate, centrifuging the filtrate to obtain a supernatant liquid, subjecting the supernatant liquid to concentration through a rotary evaporator with the temperature being controlled to be 50 DEG C, further concentrating through a nitrogen evaporator until a yellowish-brown oil substance is obtained, dissolving the obtained yellowish-brown oil substance with an n-hexane-ethyl acetate binary mixed solvent, and performing solid-phase extraction on a normal-phase silica gel column, wherein the solid-phase extraction includes activating the solid extraction column firstly with n-hexane the volume of which is 5 times of the volume of the column, balancing with the n-hexane the volume of which is 3 times of the volume of the column, feeding a sample liquid wi